兩只克隆猴在中國(guó)誕生

Scientists in China have successfully cloned two monkeys, the latest step in the country’s ambition to become a global scientific leader.

中國(guó)科學(xué)家成功克隆出兩只猴子，向著成為全球領(lǐng)先科學(xué)大國(guó)的雄心勃勃的目標(biāo)邁出最新一步。

The genetically identical long-tailed macaques born in Shanghai last month, called Zhong Zhong and Hua Hua, are the first primate clones produced through the method that made Dolly the sheep in Scotland in 1996. Their names are a play on the phrase Zhonghua, which means Chinese nation or people.

這兩只基因相同的長(zhǎng)尾獼猴上月誕生于上海，名叫中中和華華，它們是自1996年第一只克隆羊“多利”(Dolly)在蘇格蘭誕生以來(lái)，用同樣方法克隆出來(lái)的首批靈長(zhǎng)類(lèi)動(dòng)物。它們的名字寓意“中華”，即中華民族或中國(guó)人民。

Although the cloning method, known as somatic cell nuclear transfer (SCNT), is almost routine in mammals such as sheep, cattle and mice, it has been extremely difficult to replicate in primates. Previous efforts with monkeys often resulted in clones that did not develop properly as embryos or died shortly after birth.

雖然被稱(chēng)為“體細(xì)胞核移植”(SCNT)的克隆方法用于羊、牛和鼠等哺乳動(dòng)物已近乎尋常，但在靈長(zhǎng)類(lèi)動(dòng)物身上極難應(yīng)用。以前克隆猴子的嘗試往往只能得到無(wú)法正常發(fā)育的胚胎，或者在出生后很快夭折的動(dòng)物。

Now that SCNT cloning has been achieved with primates, human cloning is possible, said authors of the study, which is published in the journal Cell.

相關(guān)研究報(bào)告發(fā)表于學(xué)術(shù)期刊《細(xì)胞》(Cell)上，其作者們表示，既然靈長(zhǎng)類(lèi)動(dòng)物已經(jīng)實(shí)現(xiàn)了體細(xì)胞核移植克隆，人類(lèi)克隆是可能的。

“The technical barrier is now broken. In principle [this method] can be applied to humans,” said Mu-ming Poo, a co-author, from the Chinese Academy of Sciences Centre for Excellence in Brain Science and Intelligence Technology. But, he added, “we cloned [the macaques] to produce animal models useful for medicine, for human health. There’s no intention for us to apply this method to humans.”

“技術(shù)障礙現(xiàn)在被突破了。原則上(這種方法)可以應(yīng)用于人類(lèi)，”來(lái)自中國(guó)科學(xué)院腦科學(xué)與智能技術(shù)卓越創(chuàng)新中心(CEBSIT)的聯(lián)名作者蒲慕明(Mu-ming Poo)表示。但他補(bǔ)充稱(chēng)：“我們克隆(這些獼猴)以培育用于醫(yī)學(xué)、服務(wù)于人類(lèi)健康的動(dòng)物模型。我們無(wú)意將這種方法應(yīng)用于人類(lèi)。”

SCNT involves replacing the nucleus of an egg cell with DNA from a differentiated body cell such as skin. This reconstructed egg develops into a clone of the DNA donor.

體細(xì)胞核移植涉及用來(lái)自分化的體細(xì)胞(如皮膚)的DNA取代卵細(xì)胞的細(xì)胞核。經(jīng)過(guò)重建的卵細(xì)胞發(fā)育成DNA供體的克隆。

In 1999 US researchers at the Oregon National Primate Research Center produced a live cloned rhesus monkey, named Tetra, through embryo splitting — a simpler process that occurs naturally in identical twins but cannot generate more than four clones at a time.

1999年，美國(guó)俄勒岡國(guó)家靈長(zhǎng)類(lèi)動(dòng)物研究中心(Oregon National Primate Research Center)的研究人員利用胚胎分裂技術(shù)(一種更簡(jiǎn)單的過(guò)程，在同卵雙胞胎中自然發(fā)生，但無(wú)法一次產(chǎn)生4個(gè)以上的克隆)，成功克隆出一只名叫Tetra的恒河猴。

In principle, SCNT can produce a larger number of clones from the same donor, which could help medical research by generating genetically uniform groups of monkeys with specific characteristics.

原則上，體細(xì)胞核移植可從相同的供體產(chǎn)生更大數(shù)量的克隆，有望通過(guò)產(chǎn)生具有特定特征的基因一致的猴群來(lái)幫助醫(yī)學(xué)研究。

The team at the Chinese Academy of Sciences’ Institute of Neuroscience in Shanghai failed in its efforts to produce healthy clones from adult cells but succeeded with fibroblasts — connective tissue cells — from a macaque foetus.

位于上海的中國(guó)科學(xué)院神經(jīng)科學(xué)研究所的研究小組未能從成體細(xì)胞獲得健康的克隆，但用一個(gè)獼猴胎兒的成纖維細(xì)胞(結(jié)締組織細(xì)胞)取得了成功。

“The researchers not only replace the DNA sequence but also make sure the sequence expression of certain genes and the amount of that expression are co-ordinated so they are in the right place at the right time,” said Gang Fang at New York University Shanghai. “That’s quite difficult.”

“研究人員不僅要替換DNA序列，還要確保某些基因的序列表達(dá)以及表達(dá)的數(shù)量協(xié)調(diào)一致，使它們?cè)谡_的時(shí)間到達(dá)正確的位置，”上海紐約大學(xué)(New York University Shanghai)的方剛表示。“這種操作的難度很大。”

Zhong Zhong, eight weeks, and Hua Hua, six weeks, are apparently developing normally. The Shanghai team expects more macaque clones to be born over the coming months.

8周的中中和6周的華華顯然在正常發(fā)育。上海的研究團(tuán)隊(duì)預(yù)計(jì)未來(lái)幾個(gè)月會(huì)有更多克隆獼猴誕生。

The study raises concerns about animal ethics in China, which has less rigid testing regulations than many other countries, though the researchers followed guidelines for animal research set by the US National Institutes of Health. Dr Poo is encouraging scientists internationally to discuss the issue. “We are very aware that future research using non-human primates anywhere in the world depends on scientists following very strict ethical standards,” he said.

這項(xiàng)研究令人關(guān)注中國(guó)的動(dòng)物倫理學(xué)，中國(guó)的試驗(yàn)法規(guī)沒(méi)有其他很多國(guó)家那么嚴(yán)格，盡管上海項(xiàng)目的研究人員遵循了美國(guó)國(guó)立衛(wèi)生研究院(NIH)制定的動(dòng)物研究指導(dǎo)方針。蒲慕明鼓勵(lì)各國(guó)科學(xué)家討論這個(gè)問(wèn)題。“我們非常清楚，未來(lái)在世界上任何地方利用非人靈長(zhǎng)類(lèi)動(dòng)物開(kāi)展研究，都有賴(lài)于科學(xué)家們遵循非常嚴(yán)格的倫理標(biāo)準(zhǔn)，”他表示。

The cloned macaques are examples of the way skyrocketing Chinese state funding of basic research is yielding significant advances in fields ranging from quantum computing to biology.

克隆獼猴是又一個(gè)例證，說(shuō)明中國(guó)對(duì)基礎(chǔ)研究大舉增加的撥款，正在從量子計(jì)算到生物學(xué)等各個(gè)領(lǐng)域帶來(lái)長(zhǎng)足進(jìn)展。

Chinese state-led funding of basic research — science that has no immediate commercial applications — increased to $10bn in 2015. While that number is about one-quarter of US federal spending on basic research, it represents a vast increase from the $3.9bn in total state science funding four years earlier, according to China’s National Natural Science Foundation.

在中國(guó)，國(guó)家主導(dǎo)的基礎(chǔ)研究(沒(méi)有直接商業(yè)應(yīng)用的科學(xué)研究)經(jīng)費(fèi)在2015年增至100億美元。盡管這個(gè)數(shù)字大約是美國(guó)聯(lián)邦基礎(chǔ)研究支出的四分之一，但是相比四年前39億美元的國(guó)家科研經(jīng)費(fèi)總額，這是一個(gè)巨大增加(數(shù)據(jù)來(lái)自中國(guó)國(guó)家自然科學(xué)基金會(huì))。